Career Center

Memorial Sloan Kettering Cancer Center (MSK) is one of the world’s premier cancer centers, committed to exceptional patient care, leading-edge research, and superb educational programs. The blending of research with patient care is at the heart of everything we do. The institution is a comprehensive cancer center whose purposes are the treatment and control of cancer, the advancement of biomedical knowledge through laboratory and clinical research, and the training of scientists, physicians and other health care workers.

The Gene Editing and Screening (GES) Core Facility is recruiting a Research Assistant to provide small molecule high throughput screening (HTS) and high content cell-based screening (HCS) for researchers at MSKCC and other academic clients.  Arrayed small molecule and siRNA libraries, along with pooled functional genomics CRISPR-Cas9 and shRNA libraries, are offered for such screens. Core services include: assay development, performing large screens, data analysis, hit calling, confirmation and validation of hits, statistical calculations, dose response curves, drug and radiation synergies, synthetic lethality, and signaling pathway associations.

Responsibilities:

• Develop cell-based and non- cell-based assays for HTS and HCS screens
• Perform arrayed small molecule screening experiments, full genome siRNA knockdown and CRISPR-Cas9 KO in a 96/384 well high-throughput screening format
• Acquire large-scale image / screening data and perform Hiseq deconvolution analysis
• Collect and enter data, assist in analyzing data, preparation of reports and manuscripts.
• Maintain and develop protocols for laboratory automation including chemical inventory
• Attend and participate in project meetings, assist in preparing and conducting experiments following established procedures or protocols, summarize results of experiments performed, and assist in interpreting the results of studies
• Assist with maintaining, testing and/or implementation of robotics and automation

Education and experience:

• B.S., M.S. or Ph.D. in biology (molecular, cell, pharmacology) or related field required
• Proven hands-on experience with large scale sterile cell culture required
• At least 1-3 years of experience in running microscopy-based studies
• Some experience in advanced image analysis, RNAi and CRISPR-Cas9 techniques
• Management of laboratory robotic equipment and high-end instrumentation

Technical Knowledge and Competencies:

• Experience with HCS and HTS assay development (biochemical/ cell-based) required

• Capacity to handle multiple tasks and competing demands under tight timeframes
• Ability to work under pressure, manage competing deadlines and priorities in a fast-paced research institute environment; respect project confidentiality
• Ability to summarize detailed issues in a concise and structured manner
• Ability to understand and carry out instructions; solve problems in complex situations
• Ability to interact w/ all levels of staff in a professional manner
• Must demonstrate excellent interpersonal and communication skills
• Excellent verbal and written communication skills required
• Capacity to exhibit adept multi-tasking and organizational skills required
• Willingness to work independently and in a collaborative team environment