ChristianaCare's Gene Editing Institute has an exciting opportunity to join their team as a Staff Scientist II and utilize your experience and knowledge to work with a cross-functional team of cell and molecular biologists.
The successful candidate is expected to carry out the assigned projects mostly independently. Primary responsibilities include but are not limited to developing and optimizing CRISPR technology for mammalian cell engineering in vivo and in vitro.
Independently maintain various types of adherent and suspension mammalian cell cultures in a sterile environment
Design and optimize CRISPR components including gRNAs and HDR templates
Perform transfection of mammalian cells utilizing nucleofection, lipofection and other methods
Evaluate the transfections using cell counting and viability assessment
Single-cell sorting by FACS and limiting dilutions
Bachelors degree in biological sciences or related field required with at least three to seven years of work experience or Masters degree with less experience in a biotech setting would be considered.
Extensive experience in mammalian cell culture. Experience in assay development for mammalian cells. Knowledge in primary cell culture.
Experience with managing multiple projects simultaneously.
Knowledge in CRISPR genome engineering.
Internal Number: JR57366
About ChristianaCare Health System
ChristianaCare Health System is headquartered in Wilmington, Delaware and is one of the country's largest health care providers, ranking 21st in the nation for hospital admissions. Christiana Care is proudly a Nurse Magnet recognized institution. Christiana Care Health System is also one of the largest health care providers in the mid-Atlantic region, serving all of Delaware and portions of seven counties bordering the state in Pennsylvania, Maryland and New Jersey. A not-for-profit, non-sectarian health system, Christiana Care includes two hospitals with more than 1,100 patient beds, and is a major teaching hospital with two campuses. Christiana Care is continually recognized for excellence on a regional and national level. Our role in the community is expressed in the Christiana Care Way: "We serve our neighbors as respectful, expert, caring partners in their health. We do this by creating innovative, effective, affordable systems of care that our neighbors value."Christiana Care is a great place to work because we value diversity and recognize it to be a core part of our success. Because of the diversity of our employees, affiliated health professionals and volunteers, we are ...positioned to meet the unique needs of our patients and community. We acknowledge and celebrate the uniqueness and talent of each employee. Because of our talented workforce we are able to provide a quality healthcare experience to our patients and community. We strive to create an inclusive environment in which individual diversity can be leveraged and thrive. Christiana Care Health System is an equal opportunity employer, firmly committed to prohibiting discrimination, whose staff is reflective of its community and considers qualified applicants for open positions without regard to race, color, sex, religion, national origin, sexual orientation, genetic information, gender identity or expression, age, veteran status, disability, pregnancy, citizenship status, or any other characteristic protected under applicable federal, state, or local law.