The Wang Lab at the Division of Pulmonary Medicine at Boston Children's Hospital is seeking a highly motivated Research Assistant to support our basic research portfolio. The lab uses induced pluripotent stem cell technology and gene-editing techniques of CRISPR-Cas9 to study pediatric lung diseases, focusing mainly on cystic fibrosis. Successful candidate will also be working on one or more of the following projects: 1) Modeling various phenotypes of cystic fibrosis with iPSC-derived lung platform 2) Characterizing the function of iPSC-derived pulmonary ionocytes, 3) assessing how CF airway respond to SARS-CoV-2. The last project (3) is a joint collaboration with the BLS3 and BLS4 laboratories at the National Emerging Infectious Disease Laboratories (NEIDL), and candidate will be delivering cell cultures (prior to infection with the virus) to the NEIDL laboratories for SARS-CoV-2 infection by NEIDL trained staff. Candidate will then process safely-treated, non-infectious samples for down-stream analysis, and should not have any direct contact with live SARS-CoV-2 virus. The candidate will also have the opportunity to interact with world-class collaborators at the Harvard campus as well as outside institutions including Boston University.
This Research Assistant I will:
According to prescribed protocols and assigned schedules, perform a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy
Assist in troubleshooting procedures
Perform the following techniques typically required for the laboratory/study:
Perform directed differentiation of iPSCs to various tissue lineages, mainly lung epithelium (proximal airway, distal lung) and immune cells
Perform PCR, RT-PCR, and other common molecular biology techniques setup
Perform flow cytometry and cell-sorting
Perform immunohistochemistry
Perform microscopy, including confocal
Maintain records of results and methodologies in a log of work performed
Maintain equipment, lab area and supplies in a neat professional manner
Sterilize and clean glassware, prepare media and other materials using appropriate procedures
Inventory supplies, alert supervisor to needed supplies, or mix standard reagents
Attend courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials
Carry out all laboratory activities in compliance with applicable regulations and protocols
Conduct literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.
Attend courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information
To qualify, you must have:
BS in Biology, Molecular Biology, Biochemistry or related field
Wet bench experience is desirable
Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles
During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.
BCH offers competitive compensation and benefits.
BCHis an Equal Opportunity/Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability
Internal Number: 3118651
About Boston Children's Hospital
Boston Children's Hospital is a 395-bed comprehensive center for pediatric health care. As one of the largest pediatric medical centers in the United States, Children's offers a complete range of health care services for children from birth through 21 years of age.
