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The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.
The Department of Biochemistry has an outstanding opportunity for a Research Scientist/Engineer 1.
RESPONSIBILITIES: 1) General lab organization. This includes ordering and stocking supplies, maintaining equipment, organizing chemical and biological stocks, arranging lab schedules, and interfacing with supply vendors 2) Protein expression, purification, and characterization. Initially working on the enzyme PRP synthase, including disease related mutants, but likey to expand to other enzymes and cytoskeletal elements. This includes generating expression constructs using basic molecular biology techniques, chromatographic protein purification, measuring activity in multiple enzyme assays, and determining structures by negative stain and cryo-electron microscopy.
REQUIREMENTS: •Bachelor's Degree in Biochemistry or related field + 3 months or more years of experience in related field. •Familiarity with protein expression and purification techniques, ability to troubleshoot protein purification, knowledge of basic molecular biology techniques related to cloning, knowledge of basic microbiology techniques to grow and propagate bacterial strains for protein expression, excellent organizational skills, knowledge of fundamental concepts in biochemistry including enzyme kinetics and ligand binding.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
DESIRED EXPERIENCE •Familiarity with electron microscopy imaging or X-ray crystallography, ability to perform and troubleshoot enzyme assays.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Workforce Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.
