The Technician B will provide research support for the Reilly Laboratory in the Cardiometabolic Genomics Program focusing on RNA sequencing, single cell RNA profiling, cell biology, and molecular biology of cardiometabolic genomics and biology. The Reilly lab performs human translational studies, gene-editing in human induced pluripotent stem cell models, single cell profiling, transcriptomics, in vitro experiments and animal model work focus on cardiometabolic disorders including atherosclerosis, adipose biology and glucose homeostasis, innate immunity in metabolism, and lipoproteins.
Organization and preparation of the research lab materials
Setting and calibrating equipment and carrying out fully planned experiments
Preparation of solutions and common reagents
Cellular and molecular assays(e.g., transformation, miniprep, maxiprep, RNA, DNA and protein preparation, cDNA synthesis, qRTPCR, Transfection, packaging of viral vectors, ELISA, Western Blotting etc.),
Utilizing microscopic techniques and flow cytometry for characterization of primary cells
Interpretation of data and keeping records of results
Preparation of materials and executing protocols toward RNA profiling of myeloid cells, vascular smooth muscle cell experiments, and general molecular biology procedures.
Perform other responsibilities as directed
Requires a bachelor's degree or equivalent in chemistry, biology, physics or related science or equivalent combination of education, training and experience.
Applicant should have excellent organizational skills, willingness to work as a team under guidance with certain independence is expected, familiar with cell biology and molecular biology techniques, and must be willing to work with human materials, live animals and derived humans.
Prior lab experience with tissue culture and cell biology techniques
The candidate selected for this position is required to undergo a drug screening test prior to a final offer of employment being made
Participation in the Medical Surveillance Program
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 508775
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.