The Stevens lab leverages a wide range of techniques across a variety of cell and animal model systems, as well as postmortem human tissues, to understand brain development and disease at a molecular and cellular level, focusing on interactions between neurons and microglia, the brain's resident immune cells, and the impacts of disease risk factors on these cellular processes. The lab collaborates closely across neurobiology, genetics, stem cells, and therapeutics groups at Boston Children's, Harvard Medical School, Broad, and other area institutions to develop and apply technologies and assays to these questions. A successful applicant will contribute to multiple teams and projects, with a particular focus on supporting cutting-edge gene expression technologies such as single-cell RNA-seq and spatial transcriptomics.
This Research Assistant I will be responsible for:
Performing a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy. Assists in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.
Performing the following techniques typically required for the laboratory/study: Maintains records of results and methodologies in a log of work performed. Maintains equipment, lab area and supplies in a neat professional manner. Sterilize and clean glassware, prepare media and other materials using appropriate procedures. Inventories supplies alerts supervisor to needed supplies, or mixes standard reagents. Processes order for supplies and/or animals.
Attending courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials. Carries out all laboratory activities in compliance with applicable regulations and protocols.
Conducting literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I. Attends courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.
Contributing to the team within and between departments and organizations.
Process cells or tissue for transcriptomic analysis (e.g. single-cell or single-nucleus dissociation, capture, and sequencing library preparation).
Prepare and slice brain tissue, make slides, and perform immunostaining or other histology.
Perform basic fluorescence microscopy and image analysis.
Attend and present at small group, full lab, and collaborating lab meetings, and contribute to experimental design and brainstorming.
B.A./B.S. Degree in Biological Sciences, Biochemistry, Chemistry, or related discipline, preferably with a GPA of >3.5.
Extensive undergraduate hands-on research lab experience.
Willingness to work with animals and human tissues.
Strong organizational skills, work ethic, ability to multi-task and meet deadlines.
Ability to follow multi-step protocols and standard operating procedures.
Ability to work independently as well as in a team, in a productive, goal-oriented manner.
Willingness to commit to two years.
A CV and cover letter explaining your qualifications, interests, and career goals.
Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.
Boston Children's Hospital offers competitive compensation and unmatched benefits.
Boston Children's Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.
Internal Number: 3059638
About Boston Children's Hospital
Boston Children's Hospital is a 395-bed comprehensive center for pediatric health care. As one of the largest pediatric medical centers in the United States, Children's offers a complete range of health care services for children from birth through 21 years of age.