This is for a technician position in the Rocklin Lab in the Pharmacology Department, Feinberg School of Medicine. The lab is relatively new, having opened in March 2019. The focus of the lab is high-throughput computational protein design and experimental protein biophysics. We use a variety of techniques including recombinant protein production, protein mass spectrometry, in vitro display selections, and computational methods. See www.rocklinlab.org for more details.
The Research Technologist 2 conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.
The position can begin as early as September 2020, although the actual start date can be flexible.
The responsibilities are a mix of lab management (25%) and supporting a range of experimental projects in the lab (75%). Depending on workload and skill level, experimental duties can also grow to include an individual research project.
Lab technicians are full scientific members of the group. They attend seminars, join journal clubs, and present in our weekly group meetings along with students and postdocs.
Lab management duties include:
Obtain quotes for equipment and reagents, negotiating with vendors as needed
Installing equipment and arranging equipment repair as needed
Maintaining lab inventory
Training new lab members in experimental techniques
Assisting with ordering supplies and shipping
Experimental duties include:
Recombinant protein production from E. coli, including size-exclusion chromatography by FPLC
Standard molecular biology methods, including qPCR
Amplicon library preparation for next-generation sequencing
Assisting with protein LC-MS and hydrogen exchange experiments (experience with protein LC-MS is beneficial but not required).
Following protocols, designs, sets-up and conducts basic experiments.
Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy
Using a fully functioning level of skill, completes the following activities:
DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Library preparation for next-generation sequencing
Cell culture, cell isolation
Other (please specify):
Potentially: operation of LC-MS, mass spectrometry sample preparation
Maintains detailed records of results which may include collecting, extracting & entering data.
Manipulates, interprets &/or presents results.
Prepares charts & graphs for presentations & publications.
Performs scientific literature searches in support of research.
Completes portions of grant applications, manuscripts & publications.
Tracks & summarizes lab supply expenses.
Verifies accuracy of invoices.
Orders &/or maintains inventory of reagent, mice &/or lab supplies.
Minimum Qualifications: (Education, experience, and any other certifications or clearances)
Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience.
Minimum Competencies: (Skills, knowledge, and abilities.)
Demonstrated laboratory experience;
Demonstrated ability to follow detailed instructions as required by research protocols;
Demonstrated computer skills, including spreadsheets and basic computations;
Attention to detail and accurate record keeping;
Ability to work independently and in a team environment;
Preferred Competencies: (Skills, knowledge, and abilities)
Note: applicants are NOT expected to possess all of these skills. Please apply if you have experience in a few of these areas. This is a list of relevant skills, not required skills.
Enthusiasm for protein design, starting a new lab, and creating a positive lab culture.
Protein expression in E coli, or other host
Protein purification by IMAC
Use of Biorad or Akta FPLC for protein purification, size-exclusion chromatography
Protein gel electrophoresis & western blotting
PCR and quantitative PCR
DNA gel electrophoresis
Deep sequencing library preparation
Analysis of deep sequencing data
Protein mass spectrometry, proteomics, LC-MS
Waters Synapt G2Si mass spectrometer
Hydrogen exchange mass spectrometry of proteins
Experience with any display technology such as phage display, yeast surface display, mRNA or ribosome display
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Internal Number: 39051
About Northwestern University
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highly collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.