| Department: MED-Urology
Salary/Grade: NEX/11 Job Summary: This role conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices Specific Responsibilities: - Following protocols, designs, sets-up and conducts basic experiments.
- Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.
- Molecular Biology :
- DNA, RNA, Protein isolation, purification and expression, Western blotting, PCR, RT-PCR, Chromatin Immunoprecipitation, CO-Immunoprecipitation.
- Plasmid preparation, Cloning, mapping and sequencing of DNA, Recombinant virus production and purification
- Cellular Biology:
- Cell culture, cell isolation
- Functional studies
- Transfection
- Histology:
- Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
- Immunohistochemistry
- Immunology:
Administration - Maintains detailed records of results which may include collecting, extracting & entering data.
- Interprets &/or presents results.
- Prepares charts & graphs for presentations & publications.
- Performs scientific literature searches in support of research.
- Participates/presents in journal clubs.
- Completes portions of grant applications, manuscripts & publications.
Finance - Tracks & summarizes lab supply expenses.
- Verifies accuracy of invoices.
- Orders &/or maintains inventory of reagent, mice &/or lab supplies.
Supervises Performs other duties as assigned. Minimum Qualifications: - Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience.
- Demonstrated laboratory experience;
- Demonstrated ability to follow detailed instructions as required by research protocols;
- Demonstrated computer skills, including spreadsheets and basic computations;
- Attention to detail and accurate record keeping;
- Ability to work independently and in a team environment;
- Manual dexterity.
Preferred Qualifications: - Bachelor’s degree.
- DNA/RNA isolation. Transformation and plasmid isolation.
- Western blots
- qPCR and PCR
- Cell culture and drug treatments
- Immuno precipitation and chromatin immune precipitation
- CRISPR/CAS9 (bonus)
- Cloning
- Transfection (using siRNAs or plasmids)
- Microscopy
- Computer skills (power point/excel/word). Illustrator and Photoshop will be a bonus.
As per Northwestern University policy, this position requires a criminal background check. Successful applicants will need to submit to a criminal background check prior to employment. Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
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