This role conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices
Specific Responsibilities:
Following protocols, designs, sets-up and conducts basic experiments.
Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.
Molecular Biology :
DNA, RNA, Protein isolation, purification and expression, Western blotting, PCR, RT-PCR, Chromatin Immunoprecipitation, CO-Immunoprecipitation.
Plasmid preparation, Cloning, mapping and sequencing of DNA, Recombinant virus production and purification
Cellular Biology:
Cell culture, cell isolation
Functional studies
Transfection
Histology:
Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Immunology:
Flow cytometry
Administration
Maintains detailed records of results which may include collecting, extracting & entering data.
Interprets &/or presents results.
Prepares charts & graphs for presentations & publications.
Performs scientific literature searches in support of research.
Participates/presents in journal clubs.
Completes portions of grant applications, manuscripts & publications.
Finance
Tracks & summarizes lab supply expenses.
Verifies accuracy of invoices.
Orders &/or maintains inventory of reagent, mice &/or lab supplies.
Supervises
Trains students.
Performs other duties as assigned.
Minimum Qualifications:
Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience.
Demonstrated laboratory experience;
Demonstrated ability to follow detailed instructions as required by research protocols;
Demonstrated computer skills, including spreadsheets and basic computations;
Attention to detail and accurate record keeping;
Ability to work independently and in a team environment;
Manual dexterity.
Preferred Qualifications:
Bachelor’s degree.
DNA/RNA isolation. Transformation and plasmid isolation.
Western blots
qPCR and PCR
Cell culture and drug treatments
Immuno precipitation and chromatin immune precipitation
CRISPR/CAS9 (bonus)
Cloning
Transfection (using siRNAs or plasmids)
Microscopy
Computer skills (power point/excel/word). Illustrator and Photoshop will be a bonus.
As per Northwestern University policy, this position requires a criminal background check. Successful applicants will need to submit to a criminal background check prior to employment.
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Internal Number: 39016
About Northwestern University
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highly collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.
