The Dewar lab in the Department of Biochemistry at Vanderbilt University is seeking a talented biochemistry research assistant to join our group. This individual will work closely with our team of students and post-docs to investigate mechanisms of DNA replication and DNA repair. This position is wellsuited applicants with an interest in basic cellular mechanisms and/or cancer biology.
The Dewar Lab in the Department of Biochemistry at Vanderbilt University is seeking a hard-working and enthusiastic molecular biology/biochemistry research assistant to aid in the lab's studies of DNA replication and repair. This basic science work is highly relevant to cancer biology. The Dewar lab relies heavily on biochemistry and molecular biology, and extensively employs an in vitro DNA replication system derived from frog egg extracts. See Dewar et al, 2015, Nature and Dewar et al, 2017, Genes Dev, Heintzman et al 2019, Cell Rep, for examples of the type of research that will be undertaken. The Dewar lab is a mid-sized lab seeking a research assistant who will spend most of their time on research, with some effort put towards maintaining lab infrastructure. The new research assistant will be directly mentored and instructed by the Principle Investigator, Dr Dewar, in planning, conducting, and presenting research. It is expected that the new research assistant will produce enough data for a first author publication within 2 years of joining the lab. We have a strong training record and our previous research assistant left to attend graduate school in the biomedical sciences. Overall, this is an excellent opportunity for anyone seeking to expand their research skills or embark on the next stage of their research career.
Duties and Responsibilities
Assists in Research and Development:
Uses agarose gels to analyze radioactive DNA intermediates of DNA replication and repair
Uses protein gels and Western blotting to analyze proteins bound to DNA during different stages of DNA replication and repair
Designs and clones DNA templates for in vitro replication assays
Expresses and purifies proteins in bacteria and insect cells
Tests antibodies by immunoprecipitation and western blotting
Establishes and maintains human cell lines, and perform basic cell culture experiments
Maintains the lab's frog (Xenopus laevis) colony
Organize and maintain a healthy frog colony
Induce egg laying by injecting frogs with hormones
Collect eggs for lab members' experiments
Performs general lab and administrative duties:
Prepare buffers and reagents.
Other tasks as directed by supervisor
Internal Number: 2000543
About Vanderbilt University
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