Rutgers, The State University of New Jersey is seeking a Research Associate II for the Center for Advanced Biotechnology & Medicine (CABM) at the Rutgers Robert Wood Johnson Medical School.
The Research Associate II is responsible for the conduct of highly specialized and technical research in the field of cancer research. The Research Associate II will focus on the study of anti-tumor effects of probiotics on breast cancer, and to analyze whether probiotics enhance chemotherapy and reduce side effects. Will perform tumor cell injection, daily oral gavage, tumor size measurement, monitoring lung metastasis by IVIS imaging, scoring mouse stool consistency, body weight measurement, blood collection, necropsy, tissue collection (primary tumor, lung, intestine, spleen), FACS analysis of splenocytes from the collected spleens for myeloid-derived suppressor cells (MSDC) and potentially other markers, H&E staining of tumor sections, histological staining for mucositis in the intestine, immunohistochemical staining of tumor sections, for a number of markers that are related to cell growth, apoptosis, and immune functions, serum analysis for a number of cytokines and other factors, and potential analysis of tumor lysates for certain proteins and RNA. Mouse model of breast cancer will be used and a large number of mice will be involved in this project.
Among the key duties of this position are the following:
Injection of breast tumor cells subcutaneously into the right upper mammary gland of mice.
Daily oral gavage of mice (including weekends and holidays). Once started, an experiment can be up to 7-8 weeks.
Tumor size will be measured at least three (3) times a week. Requires daily measurements, calculating tumor volumes, plot tumor volumes, and tumor weight will be measured at mouse sacrifice.
For the chemotherapy experiments, need to score mouse stool consistency and measure body weight daily. Histological analysis of villus height and crypt depth will also be performed to assess mucositis.
Heart puncture to collect blood immediately after a mouse is euthanized. At necropsy, collect tissues, preserve primary tumor, lung and intestine with appropriate methods.
Minimum Education and Experience:
Ph.D. Degree or equivalent in Cancer Biology or a relevant science, or a Master’s Degree in a relevant science plus three (3) years of related research experience.
Extensive mouse work experience.
Mouse work experience must include oral gavage, injections, collection of blood and tissue samples.
Required Knowledge, Skills, and Abilities:
Must be able to work with others for collaborative analyses.
Must be able to keep very good records of all experiments.
Cancer research experience strongly preferred.
Experience in tumor size measurement, IVIS imaging, FACS analsis, H&E staining, and immunohistochemical (IHC) staining strongly preferred.
Familiar with the basic statistical methods to calculate whether the experimental results are statistically significant.
Physical Demands and Work Environment:
Laboratory environment. Universal precautions are mandatory.
This is a grant-funded position contingent upon the availability of funds to support it.
Posting Number: 20ST1076
Location: Downtown New Brunswick
Internal Number: 115938
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.