Participates in design and execution of research lab projects. Conducts experimental tests and procedures and processes and analyzes data. Reviews and revises existing research methodologies and may develop new methodologies, mathematical and/or computer models for predicting data. May ensure overall lab compliance with all associated rules and regulations.
Specific Responsibilities:
The primary focus of the Horiuchi lab is to study the mechanisms of tumor progression in breast cancer and to identify novel targeted treatment strategies. We are particularly interested in understanding the cellular events that influence clinical outcomes in patients with triple-negative breast cancer (TNBC). Candidates are expected to be fluent in fundamental molecular and cell biological techniques and capable of functioning in a fast-paced environment. Additionally, strong organizational skills and operational excellence are an absolute must.
Following overall research objectives, independently designs, plans, conducts and troubleshoots experiments.
Evaluates protocols and data obtained from groups of experiments and recommends changes to processes, procedures and techniques to improve quality and validity of results based upon overall project objectives.
Using an advanced level of skill, completes the following activities:
Molecular Biology:
DNA, RNA, Protein isolation, purification and expression
Western blotting
PCR, RT-PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Cellular Biology:
Cell culture, cell isolation
Functional studies
Transfection
Histology:
Immunohistochemistry
Microscopy
Immunology:
Flow cytometry
ELISA, Elispot assay
Animal:
Dissections
Blood collection
Surgical procedures
Other (please specify):
Administration
Observes experiments, collects, records, summarizes, analyzes and interprets research data.
Collates relevant mathematical results.
Prepares complex tables reflecting relationships of multiple tests.
Draws scientific conclusions.
Writes portions of grant applications.
Co-authors scientific papers.
Finance
Negotiates purchases with vendors.
Verifies accuracy of invoices.
Orders and/or maintains inventory of reagent, mice and/or lab supplies.
Supervision
Trains and directs other lab EEs and/or students in research techniques and procedures.
Miscellaneous
Performs other duties as assigned.
Minimum Qualifications:
Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience required; OR
Successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.
Minimum Competencies: (Skills, knowledge, and abilities.)
A solid working knowledge of molecular & cell biology techniques including but not limited to nucleic acid/protein handling, molecular cloning, gene expression/silencing/editing (CRISPR), cell culture
Multicolor (4+) flow cytometry
Animal (mice) handling
Preferred Competencies: (Skills, knowledge, and abilities)
An advanced training in immunology (with emphasis on the biology of T cells and MDSCs).
Fluorescence microscopy (confocal)
Prior in-depth training in cancer research involving rodent animal models (e.g., transgenic/syngeneic/PDX) is desired
A solid understanding of fundamental statistical analysis methods
Northwestern University is an Equal Opportunity, Affirmative Action Employer of all protected classes, including veterans and individuals with disabilities. Women, racial and ethnic minorities, individuals with disabilities, and veterans are encouraged to apply. Hiring is contingent upon eligibility to work in the United States.
Internal Number: 38806
About Northwestern University
Northwestern University is a major private research university with 12 academic divisions located on three campuses in Evanston, Chicago, and Education City in Doha, Qatar. We have approximately 2,500 full-time faculty members, 17,000 graduate and undergraduate students, and over 5,700 full and part-time staff. Northwestern University combines innovative teaching and pioneering research in a highly collaborative environment. It provides students and faculty exceptional opportunities for intellectual, personal and professional growth.
