A research technician position is available in Dr. Andrew Sproul's lab in the Taub Institute for Research on Alzheimer's Disease and the Aging Brain at Columbia University Medical Center. Dr. Sproul directs the Stem Cell and Cellular Models Platform, a rapidly evolving resource for Taub researchers that creates stem cell models of Alzheimer's disease and other neurodegenerative and neurological disorders. Dr. Sproul's lab uses CRISPR/Cas9 genome editing technology to introduce mutations into control pluripotent stem cell (PSC) lines to create allelic series of disease risk factors in the same genetic background. In addition, the Platform differentiates PSCs into various disease-relevant CNS cell types which can be further manipulated by viral-mediated overexpression or siRNA knockdown. This includes neurons, astrocytes, microglia, and brain microvascular cells, as well as multicellular organoids. This work is highly collaborative in nature with other research groups within the Taub Institute. The position will also involve aiding Director Andrew Sproul's own research on Alzheimer's disease PSC models and improving stem cell differentiation methodology.
Duties will include significant cell culture work as well as assays such as immunocytochemistry, PCR, lentivirus production, and DNA/RNA isolation among other molecular techniques. Other responsibilities will include maintaining lab equipment and supplies.
Maintain stem cell cultures and conduct differentiation experiments.
Genome-edit stem cell lines using CRISPR/Cas9 technology.
Conduct molecular biology assays: i.e. DNA/RNA prep, PCR, immunostaining, etc.
Assist with the ordering and tracking of laboratory supplies, general lab organization and upkeep, re-stocking common reagents and solutions, and maintaining lab databases.
Requires a bachelor's in chemistry, biology, physics or related science or equivalent combination of education, training and experience.
Previous cell culture experience, and preferably some molecular biology experience.
Prior technical or undergraduate research experience is required.
The applicant must be able to multitask effectively for different projects and have strong organizational skills.
The ideal candidate will be engaged in the scientific questions being investigated as well as proactive in helping to maintain an organized and effective laboratory environment.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 506926
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.