The Yang Lab at Rutgers New Jersey Medical School seeks a qualified and passionate Lab Technician for systems biology and immunology research. Our lab brings together diverse experimental and computational activities to understand the molecular mechanisms underlying the pathogenesis and treatment of chronic and infectious diseases.
This position will focus on cell signaling and metabolism in innate immune cells as they pertain to bacterial infections and antibiotic treatment. The initial contract is for 1 year and may be renewable annually, based on satisfactory performance. This position requires a Master's in a biological and/or computational discipline at the time of appointment with at least 1 year experience, or Bachelor's with at least 2 years experience. This position has the opportunity to evolve into a long-term lab scientist within the lab.
The ideal candidate also possesses meaningful microbiology experience in cellular or animal studies. Programming skills (e.g., MATLAB, Python, R) are a plus, but not required, as there will be ample training opportunities in bioinformatics, network modeling and machine learning. Interest and experience in tuberculosis is also a plus, but not required.
The successful applicant will receive hands-on career mentorship and training in both experimental and computational techniques, as necessary for their work. The Yang Lab is ideally situated in the International Center for Public Health Building, home to the Center for Emerging and Re-Emerging Pathogens, Public Health Research Institute, Department of Microbiology, Biochemistry and Molecular Genetics, and Global Tuberculosis Institute at Rutgers. The successful applicant will have a breadth of access to equipment for high-throughput and live-cell assays, such as an in-lab Nikon confocal microscope, Seahorse XFe extracellular flux analyzer and a BioTek Cytation, as well as shared equipment in neighboring labs and core facilities.
To apply, please first visit the Yang Lab website (www.jasonyanglab.org) and then submit a CV including contact information for 2 references and a brief cover letter describing long-term career goals and research interests.
Application review will begin February 1, 2020, and continue until the position is filled.
Among the key duties of this position are the following:
Performs immune cell primary isolations. Performs tissue culture experiments with primary immune cells and with mammalian cells lines. Sterile technique required.
Performs immunological and molecular biology assays including, not limited to, pathogen co-culture, cytokine profiling, flow cytometry; DNA/RNA/protein purification, PCT and RT PCR, CRISPR, genetic cloning; DNA/RNA/protein gel experiments; single cell characterization by microscopy; -OMICs characterization by nextgeneration sequencing or mass spectrometry; phenotypic characterization by rodent infection models, plate-based ELISA assays.
Assists in the development and testing of new assays including chemical and genetic screens, next-generation sequencing, and/or microscopy.
Works safely in a BSL3 environment, compliant with biosafety protocols.
Assists in laboratory organization, including the ordering and stocking of supplies for laboratory efficiency and cost effectiveness. Submits purchase recommendations, to the Principal Investigator, of equipment and supplies.
Assists the Principal Investigator with the preparation of grant applications and manuscripts.
Assists the Principal Investigator in written and formal presentations to industrial collaborators and funding agencies.
Minimum Education and Experience:
Master's Degree in a relevant science plus one year of related research experience, or a Bachelor's Degree in a relevant science plus two years of related research experience.
Required Knowledge, Skills, and Abilities:
Research experience in immunological assays with mouse or human macrophages.
Molecular biology experience in DNA/RNA extraction, PCT and RT PCR, genetic cloning.
Previous experience with rodent infection models.
BSL3 experience working with virulent CDC classified tier 1 select agents and M. tuberculosis.
Programming languages and prior experience in systems biology / computational biology / data science / machine learning / bioinformatics techniques.
Previous experience with microscopy, next-generation sequencing, mass spectrometry, CRISPR experiments.
Physical Demands and Work Environment:
This position involves prolonged standing, walking and sitting, as well as use of laboratory equipment.
Must perform complex procedures and demonstrate manual dexterity.
Occasional lifting of 20 pounds to a height of 6½ feet.
Must be available to work flexible hours including weekends and holidays when necessary to meet deadlines.
Must be willing to work with rodents.
Salary: Maximum authorized hiring amount $54,613
Posting Number: 19ST5094
Location: Newark (RBHS)
Internal Number: 107680
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.