Under the general supervision of a faculty investigator or laboratory supervisor, is responsible for performing routine analyses and tests on animal, human tissue and primary cells as well as human cultured cell lines. Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience. Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention.
Specific duties & responsibilities:
Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and experiments which include: basic tissue culture and isolation and culture of cells from human and animal tissue, cell exposure assays, and basic molecular biology including Western Blotting, immunofluorescence, confocal microscopy, live imaging, qPCR and animal injections.
Detailed record keeping and maintenance of a physical or electronic lab notebook. Systematic and detailed data maintenance for future verification.
Operates basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, scintillation counter, spectrophotometer, light and confocal microscope, and utilizes laminar flow hood. In addition, will need to learn our cell exposure set up. Operates lab computers for data entry.
May utilize in vivo models in experiments. Following experimental protocols, monitors animals and maintains health records in accordance with USDA and AALAC guidelines. Sacrifices and dissects animal remains as required. Prepare tissue for study utilizing histochemical techniques.
Bleeds and injects animals according to protocols.
Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports.
Harvests primary cell cultures from blood and/or tissue specimens. Maintains primary cell lines through procedures which may include passaging, cryopreservation, transfection and/or cloning.
Maintains and makes minor adjustments to equipment, e.g. calibrating, alignment.
Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
Completes annual university biohazard/universal precaution/radiation safety training, as appropriate.
Uses sterile techniques to avoid contaminating lab experiments.
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Oversight of undergraduate students in the lab.
Minimum qualifications (mandatory):
BS/BA, or equivalent, in biological sciences, chemistry, or physics
Six months of post-undergraduate experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements.
JHU Equivalency Formula: Additional related experience may substitute for required education, to the extent permitted by the JHU equivalency formula
Special knowledge, skills, and abilities:
Scope of Responsibility: Knows the informal policies, procedures and practices necessary to conduct the normal function of a specific section, unit, or work area. Is aware of the role of the position and its potential impact on the working unit.
Decision Making: Carries out duties and responsibilities with limited supervision. Makes decisions and establishes work priorities on essentially procedure-oriented operations.
Communication: Exchanges routine information in an appropriate manner requiring good oral and written communication skills.
Any specific physical requirements for the job:
Work produced is subject to precise measures of quantity and quality
Work environment may include areas of unpleasant extremes of cold or heat
Biohazardous conditions such as the risk of radiation exposure, fumes or airborne particles, and/or toxic or caustic chemicals may be present in this work environment which mandate attention to safety considerations
Near vision to see objects clearly within 20 inches
Sharp focus to adjust vision when doing close work that changes in distance from eyes
Full spectrum vision to identify and distinguish color
Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard
Handling by seizing, holding, grasping, turning or otherwise working with the hand or hands, but without finger dexterity
Sitting in a normal seated position for extended periods of time
Occasionally lifting, carrying objects weighing 10 lbs. or less
Occasionally pushing, pulling objects weighing 30 lbs. or less
Ability to move about
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14-$19.44 Employee group: Full Time Schedule: Monday-Friday/8:30am-5:00pm/40 hours Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10002817-SOM DOM Pulmonary Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at email@example.com. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.