The Research Assistant II will support a dynamic laboratory of about 10 people working on breast cancer and epithelial biology, that includes graduate students and postdoctoral fellows. The RA II will perform routine laboratory tasks necessary for the daily operation of the lab including ordering supplies, keeping inventory up to date, making solutions and other reagents, and maintaining laboratory equipment. The RA II will also perform experiments using cell and molecular biology techniques such as cloning, cryo-sectioning, cell imaging, antibody characterization, immunoprecipitations and blotting, protein expression, cell culture, lentivirus production, and transfections.
The research laboratory consists of a group of about 10 graduate students and postdoctoral fellows, who are pursuing cutting-edge research projects in breast cancer and stem cell biology, high-end microscopy, cancer biology and epithelial biology. The laboratory is situated in the Department of Cell and Developmental Biology, which provides a vibrant, interdisciplinary environment for cutting-edge research. CDB is an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top-ranked developmental biology program, and exceptionally strong faculty.
Duties and Responsibilities
Order supplies, keep laboratory inventory up to date, make solutions and other reagents. Work with sales representatives, obtain quotes, order equipment.
Maintain mouse colonies, breeding records, and animal protocol. Ensure that lab personnel follow protocol. Perform genotyping of mice.
Practice safety, environmental, and/or infection control methods; maintain radioactivity log and associated wipe tests as necessary.
Maintain cell cultures, reagent stocks, liquid nitrogen tanks, and freezers.
Work with lab personnel on cell and molecular biology experiments.
Sets up equipment for use in lab; ensures proper operation of equipment, and reports operating deficiencies to supervisor; provides training to incoming students and postdoctoral fellows in equipment operation.
Possibility of developing independent research project.
Profile of an Ideal Candidate
A Bachelor's degree from an accredited institution of higher education is necessary.
At least two years of experience working in laboratory is necessary.
Technical skills in basic cell and molecular biological techniques are necessary.
Experience with handling mouse colonies, breeding, and genotyping is preferred.
Experience with microscopy/FACS is preferred.
Experience with ordering, maintaining laboratory stock, dealing with vendors, and maintaining schedules is necessary.
Ability to interact with students, faculty and/or staff in a team environment is strongly preferred.
Internal Number: 1902101
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.