As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a "Great College to Work For" for four consecutive years.The University of Washington, Department of Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health, and offers an array of specialized educational training programs.UW Medicine Pathology brings together state-of-the-art technology and highly trained, world-renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Pathology faculty members also serve at Seattle Children's Hospital, the Veteran's Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic pathology.The Young Laboratory in the Department of Pathology and the Institute for Stem Cell and Regenerative Medicine has an outstanding opportunity for a full time Research Scientist/Engineer 2.This position will support research using human induced pluripotent stem cells (hiPSCs) to study genetic variation leading to the predisposition for Alzheimer's disease (AD). Responsibilities: (100%):Maintaining human pluripotent stem cell linesDifferentiation of stem cells into central nervous system cellsOptimizing protocols for cortical organoid developmentPerforming assays of cell function using immunofluorescence, flow cytometry, ELISA, and electrophysiology techniquesMolecular/biochemical characterization including DNA/RNA isolation, qRT-PCR analysis, molecular cloning, western blot and protein analysis.Assist in the training as supervision of students, postdoctoral fellows and other laboratory staff.Coordination of lab organization and function: ordering supplies, maintaining and ordering lab environment.Developing variant iPSC lines using CRISPR.Developing reporter iPSC lines using CRISPR.Minimum Requirements:Bachelor's degree in Biology, Biochemistry, Molecular Biology, Neurosciences or equivalent and at a minimum 2 years relevant experience in the fields of Biology, Biochemistry, Molecular Biology, Neuroscience or equivalent.This position requires performing laboratory research independentlyThis position requires experience with human pluripotent stem cell lines (ES or iPS)Desired:Master's degree in Biology, Biochemistry, Molecular Biology, Neurosciences plus 4 years relevant experienceExperience with central nervous system cell differentiation protocols from pluripotent stem cellsExperience with molecular cloning, DNA/RNA, and protein analysisExperience with flow cytometry and FACS analysisTerms and conditions for employment:Able to work a flexible schedule with occasional off hours and weekend work. Application Process:The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.