Job Summary/Essential Job Functions: Works under the direction of a Principal Investigator (PI) to manage the general maintenance of the laboratory, perform experiments using patch-clamp electrophysiology, molecular biology techniques, and establish zebrafish colonies. Determines the etiology of diabetic retinopathy at cellular molecular tissue level using the state-of- the-art technologies.
DUTIES AND RESPONSIBILITIES:
Performs patch-clamp experiments on isolated cells and slice preparations from retina.
Conducts experiments using biochemistry and molecular biology tools.
Establishes zebrafish colonies: sets up breeding and collects embryos.
Checks equipment weekly to ensure proper functioning; catalogs lab supplies and sterilization of solution and supplies (pipette tips, bottles, etc.); and maintains the general lab cleanliness (wash empty bottles; wipes tabletop, autoclave biohazard waste, paper towels, etc.).
Maintains daily log of imaging of embryo/adult zebrafish retina.
Performs molecular and cellular experiments.
Performs genomic DNA isolation and PCR analysis for genotyping.
Performs protein isolation and protein measurement assays.
Performs other related duties as assigned.
Internal Number: 178887
The mission of the University of Tennessee Health Science Center is to bring the benefits of the health sciences to the achievement and maintenance of human health, with a focus on the citizens of Tennessee and the region, by pursuing an integrated program of education, research, clinical care, and public service.