As one of the founding basic science departments at IU School of Medicine, the Department of Anatomy and Cell Biology has a long-standing and diverse history of anatomical science education and research dating back a hundred years. Today, modern anatomy as a scientific discipline encompasses cell and molecular biology, neuroscience, developmental biology, evolution, vertebrate paleontology, and anthropology, and this area of medicine leads the way in imaging technology and imaging-based research.
The person in this position will work as a lab technician on studies dissecting mechanisms of insulin action that regulate glucose uptake by fat and muscle cells, and defining defects contributing to insulin resistance associated with obesity and type 2 diabetes. Specifically, we focus on the mechanism of cholesterol accumulation in fat and muscle that disrupts cytoskeletal structure essential for glucose transporter GLUT4 regulation by insulin. The primary role will be assisting with ongoing studies asking novel questions about cellular cholesterol accumulation and GLUT4 dysregulation, what mechanisms are involved, and the impact of targeting this toxicity on preventing and/or improving insulin resistance.
Primary Job Duties and Responsibilities:
Take care of mouse colonies, including keeping record of the mice, breeding, genotyping, performing injections, and participate in tissue collection from animals. Follow approved IACUC protocols on file in the laboratory.
Perform in vivo testing including glucose and insulin tolerance tests. Follow protocols on file in lab.
Perform in vitro testing including subcellular fractionation, lipid assays, confocal microscopy, western blotting, PCR, and ELISA. Follow protocols on file in lab.
Make buffers and reagents for running assays for animal and cell culture studies. Follow instructions from lab protocol notebooks.
Cell culture, including thawing, expanding and freezing cell lines, drug treatment, microscopy and obtaining protein and RNA samples, DNA plasmid isolation and purification; transfections and transduction of genes into mammalian cells. Follow standard protocols on file in lab.
Bachelor’s degree in science from an accredited institution or advanced level science and two years of research experience.
Combinations of education and related experience maybe considered.
Ability to effectively communicate and exchange information.
Background in biological sciences, biochemistry, or molecular biology. Some lab experience or experience with experimental mice.
Working Conditions/Physical Demands:
Must be able to perform the essential functions of the job with or without an accommodation. This position involves work with rodents.
Equipment utilized: Some familiarity with common lab equipment, e.g. pH meters, PCR machine, scales, pipets, chemical/biological hoods.
Founded in 1820, Indiana University is the state’s public university system, comprised of two core campuses—IU-Bloomington and IUPUI—and six regional campuses located throughout the state. Combined, the total student population is more than 114,000 undergraduate, graduate and professional school students. IU is one of the largest employers in the state, with over 20,000 faculty and staff. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. Diversity and inclusion remains a core commitment to IU’s mission to cultivate an educational environment that supports equal access, participation and representation on all of its campuses and that provides educational and career opportunities for all.