As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. All of which has allowed the UW to be nationally recognized as a "Great College to Work For" for five consecutive years.The Gale laboratory at the University of Washington has a job opening for a Research Scientist 2.Our lab is a highly collaborative, fast paced research lab with over 30 members. We are part of the Department of Immunology, the Center for Innate Immunity and Immune Disease, and the Washington National Primate Research Center at the University of Washington. Our research is focused on the use of systems biology approaches to define and model virus-host interactions and the varied strategies used by viruses to evade cellular innate immune defense mechanisms and to alter programming of the adaptive immune response. Through the application of computational and bioinformatics approaches, using human samples, several different animal models including inbred transgenic mice, the collaborative cross mouse model, and non-human primates, we investigate the host response to a wide range of viral pathogens, including HIV/SIV, Zika virus, West Nile virus, and influenza virus.OVERVIEWHigh-throughput RNA expression profiling is presently the most robust and comprehensive global characterization technique to apply to a biological model. Given its broad coverage of both protein-coding RNAs and non-coding RNAs, it is also the most rapid methodology as compared to other global profiling techniques such as proteomics or metabolomics. For these reasons, global expression measurements are typically the basis for systems biology approaches in a multitude of biological contexts, including the host response to infectious agents or vaccination. The research associated with this position focuses on such high-throughput transcriptomic measurements for nonhuman primate samples from animals enrolled in priority protocols conducted by the Simian Vaccine Evaluation Unit (SVEU) and for samples from other investigator-initiated studies. The major tasks associated with this position include high-throughput RNA/DNA isolation and QC, construction of sequencing libraries (mainly mRNA-seq and totalRNA-seq libraries), and qRT-PCR assays.RESPONSIBILITIESWorking independently to perform large-scale high-throughput 16S-seq or DNA/RNA-seq assays.Constructing sequencing libraries for RNA/DNA sequencing.Large scale RNA and DNA isolation from different cell and tissue types. In most cases, this will include sample processing utilizing BSL2+ practices.Performing and optimizing molecular biology protocols.Performing qRT-PCR assays.Determining nucleic acid concentrations on research samples by spectrophotometric and other plate-based assay methods.Maintaining laboratory records and experimental results into the web-based LabKey LIMS.Managing sample acquisition from collaborating research laboratories.Maintaining sample inventory and performing sample tracking.Participation in meetings to assist in developing methodology, protocols and troubleshooting.Presenting experimental results to other laboratory members.Performing duties related to maintenance of research supplies and equipment, and other duties as requested.MINIMUM REQUIREMENTSBachelor's or Master's degree in the field of biology or chemistry, and a minimum of 2 years of post-graduate laboratory experience.Ability to work independently to perform all steps required for large-scale high-throughput sequencing assay beginning with receipt of cell or tissue lysates.Experience in large scale RNA and DNA isolation from different cell and tissue types.General knowledge of methods in molecular biology laboratories and general understanding of principles behind methods.Knowledge of procedures for handling biohazard material.Ability to work well in team.Must be organized, detail oriented, and efficient.Good communications skills required; experience with Microsoft Office Word and Excel for data reporting and experimental calculations.Equivalent education/experience will substitute for all minimum qualifications except where there are legal requirements such as license/certification/registration.DESIRED REQUIREMENTSExperience with automated liquid handling systems.Experience working with in BSL2 and BSL2+ facilities.Experience with single cell sequencing.CONDITIONS OF EMPLOYMENTMust be comfortable working with animal samples and using BSL2+ practices.Application Process:The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.