Neuroepithelial Lab is focusing on the function of neuropeptide systems in skin wound healing and skin sensory disorders. We study the role of neuropeptides and their receptors in nerve regeneration and their interactions with epithelial cells to mediate the wound healing process. The nervous system in skin interacts closely with different skin cells, such as keratinocytes, Merkel cell, and also stem cells. We have shown that neurotransmitter system and other sensory receptor system exist in skin cells and influence both skin sensation and homeostasis. The aim of this project is to study the role of neural transmitters in the skin peripheral nervous system and function of novel sensory receptors in skin using human stem cell co-culture system, molecular and cellular biology, Calcium imaging to study the function of keratinocytes and sensory neurons and their interaction. We collaborate extensively with engineers and clinicians and aim for high impact research including international space station experiments.
Job Responsibilities: Grow 3D full thickness skin model and create new 3D models with genetic modified cells and incorporate other cell types including vascular, neural and pigmented cells into the models. 40% Derive human peripheral sensory neurons from human stem cells. Study the interactions between epidermal cells and peripheral nerve fibers in co-culture. Characterize the change of the quality and function of the nerve fiber or the epithelial cells due to the presence of the other component. Experiments will involve culture of skin cells and neuronal cells and co-culture of both; calcium imaging; confocal microscopy. 30% Analyze morphology, protein and RNA expression in human skin samples. The techniques involve immunohistology, various microscopy and spectroscopy and RNA isolation and various RNA analysis methods. 20% Study the effect of different opioid ligands, and other neural transmitters on nerve development and guidance in-vitro and the changes in the interactions between epithelial cells and nerve fibers. Investigate the opioid receptor’s role in this interaction using our transgenic cells. 10%
All required qualifications must be documented on application materials.
Required Qualifications: BS/BA degree in a related field and 2 years of related experience or a combination of education and work experience to equal six (6) years. Experience in stem cell research, cellular/molecular biology, and confocal microscopy Demonstrated skills in experiment handling and design, cell culture, animal surgery Demonstrated experience working with cell lines and primary cells (preferably skin and neuronal origin) Good team player, able to think for oneself and good at troubleshooting Passionate about own work with strong work ethics.
Preferred Qualifications: Masters degree in a related field Familiarity with various light microscopy (confocal microscopy) techniques, also immuno- histochemistry Bio-engineering and experience designing new assays for co-culture system
Cell culture of human primary human skin keratinocyte, stem cell derived sensory neurons and related cells for 2D and 3D models; Molecular (RNA, DNA and Protein) and cellular analysis of activation of signaling pathway Knowledge, skills using different light source and interface design with microscopy; optical physics for novel in-vivo human studies using spectroscopy and/or in-vivo confocal microscopy; Fluorescence and confocal microscopy Able to work and trouble-shoot independently.
Internal Number: 332364
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.