UCSD Layoff from Career Appointment: Apply by 08/13/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 08/22/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, the Lab Assistant will assist the Principal Investigator in research aimed at creating anti-pathogen effector and genetic drives systems to control dengue and other vector-borne diseases using a variety of standard biochemistry and molecular biology procedures. In addition, the incumbent is responsible for the care and maintenance of fly and mosquito mutant lines; using a stereo microscope; and performing routine fly and mosquito work to include: feeding colonies (preparing food and blood feeding), sorting flies, collecting flies, setting up crosses, transferring stocks, screening flies for mutant phenotypes, etc.
Following genetic screens, the incumbent will assist senior laboratory staff with molecular biology procedures such as DNA isolation, recombination-based cDNA cloning, bacterial transformation and growth, PCR and Gibson assembly and with the microinjection of fly embryos. The incumbent will also perform routine laboratory duties including: ordering and maintaining lab equipment, supplies, and records; maintaining cleanliness of lab; preparing buffers, media, and solutions; and performing other general lab duties as needed.
Must be able to work occasional overtime (evening or weekend) based on needs of experiments.
Graduation from high school or a GED and two (2) years of laboratory experience; OR two (2) years of college including courses in biochemistry, molecular biology, biophysics, basic genetics; OR an equivalent combination of education and experience
College level course or equivalent in general laboratory techniques and molecular techniques including the ability to prepare solutions and use a pH meter
Laboratory experience with basic and general techniques in molecular biology including: DNA and RNA preparation and extraction, molecular cloning, gel electrophoresis, PCR, etc.
Proven skill to use standard lab equipment such as centrifuges, microscopes, balances, etc.
Demonstrated skill to culture and handle Drosophila flies and mosquitoes.
Demonstrated experience with basic microbiological and sterile techniques.
Proven knowledge of purchasing and reimbursement procedures. Skill in maintaining steady levels of lab supplies, maintaining purchasing records, and training others in purchasing procedures.
Strong organizational skills to keep accurate records of scientific data in a well-organized lab notebook. Skill to follow multistep assays and procedures.
Proficiency using MAC or PC computers and software, including Microsoft Office.
Excellent verbal and written communication skills. Strong interpersonal communication skills including tact, diplomacy, and flexibility with students, staff, faculty, and visitors of various cultures and backgrounds.
Knowledge of laboratory safety precautions including appropriate clothing and shoes, safe handling of chemicals, and use of protective items such as gloves and goggles, etc.
Knowledge of safety procedures using chemicals and radioactive materials. Knowledge of Material Safety Data Sheets, hazard communication and Injury and Illness Prevention Programs.
Ability to embrace and consistently uphold the UC San Diego Principles of Community for a collaborative work environment.
Must be able to work safely with lab chemicals.
Must have good eyesight (normal or corrected) and manual dexterity for fine dissection of insects.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.