The Platform was primarily created to facilitate human stem cell research in the Taub Institute. It serves as a rapidly evolving resource for Taub researchers to create stem cell models of Alzheimer's disease and other neurodegenerative and neurological disorders. To this end, the Platform uses CRISPR/Cas9 genome editing technology to introduce mutations into control PSC cell lines to create allelic series of disease risk factors in the same genetic background. In addition, the Platform differentiates pluripotent stem cell (PSC) lines into various disease-relevant CNS cell types which can be further manipulated by viral-mediated overexpression or knockdown. This includes neurons, astrocytes, microglia, and brain microvascular cells, as well as multicellular organoids.
Significant cell culture work as well as assays such as immunocytochemistry, PCR, lentivirus production, and DNA/RNA isolation among other molecular techniques.
Maintaining lab equipment and supplies.
Multitask effectively for different projects.
Aiding Director Andrew Sproul's own research on Alzheimer's disease PSC models and improving stem cell differentiation methodology.
Collaborating in nature with other research groups within the Taub Institute.
Bachelor's degree in chemistry, biology or physics or a completed animal husbandry program or equivalent in education, training and experience.
Flexible hours are a plus.
Previous Cell culture experience.
Molecular biology experience.
Strong organizational skills.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 502156
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.