A highly qualified and motivated technician is sought to join the Provenzano laboratory in the Department of Biomedical Engineering and the Masonic Cancer Center at the University of Minnesota in Minneapolis MN. Our laboratory focuses on understanding the molecular mechanisms by which the stromal extracellular matrix and stromal cell populations influence epithelial cell behavior in cancer. Fully integrating engineering and biology, we are particularly interested in how these factors influence disease progression to metastasis and resistance to therapeutic intervention in breast and pancreas cancer. To answer these fundamental questions we rely heavily on genetically engineered mouse models and transgenic mice (for examples see Nature Communications 8:14923, 2017; Cancer Cell 21(3), 418-429, 2012; American Journal of Pathology 173(5), 1551-1565, 2008). In addition, we utilize advanced quantitative imaging, tissue engineered 3D microenvironments and cell and molecular biology techniques to gain a quantitative understanding of cancer cell behavior and develop novel technologies and therapeutic strategies for cancer diagnosis and treatment. This represents an excellent opportunity for a highly motivated individual to work and learn in a tight-knit team and directly contribute to the study of relevant problems in cancer.
Mouse work (husbandry, including breeding, tagging and weening, genotyping, and performing necropsies to obtain tissues for histology and establishing cell lines (75%)
General management and organization of the laboratory (ordering, maintain lab inventories, direct others in safety, maintenance of equipment and supplies, training undergraduate students, and troubleshooting) 20%
Additional wet bench work (maintenance and study of primary cell lines and isolation of DNA, RNA, and proteins) 5%
BA/BS or a combination of related education and work experience to equal four years; applies knowledge and skill to a recurring task or activity with emphasis typically on precision and timeliness of execution.
Experience with Mouse colony management, mouse handling and husbandry, including breeding, tagging and weening, genotyping, and performing necropsies to obtain tissues for histology
Mammalian cell culture
DNA extraction and PCR analysis
Maintaining lab/procedure records
Strong troubleshooting skills
Internal Number: 332177
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.