Our laboratory in the Immunology Program at Memorial Sloan Kettering investigates immune responses to cancer, molecular mechanisms underlying tumor-induced T cell dysfunction, and new approaches for cancer immunotherapy. We have an outstanding opportunity for a Research Technician interested in projects investigating immune responses to cancers.
Managing transgenic and knockout mouse strains and carrying out genotyping by PCR and flow cytometry
Assisting lab members with experimental procedures
Analyzing peripheral blood and tissues from mice and humans by flow cytometry
Administration of cells, immunostimulatory reagents, and/or medications to mice via intraperitoneal, subcutaneous, and intravenous routes
Monitoring mice health and tumor development
Preparing DNA and RNA from primary mouse tissues and cell lines for quantitative RT-PCR analysis
Designing and carrying out cloning projects
Culturing mouse and human cell lines and carrying out in vitro transfection/transduction
General lab upkeep, including preparing reagents, ordering etc.
BS in Biochemistry or Biological Chemistry, Molecular Biology, Biophysics, or related fields
At least 1-2 years of experience working with mice, including genotyping
Experience with molecular biology including PCR and bacterial work
Demonstrated ability to hold a position of responsibility for an extended period of time
Ability to learn new techniques and work independently once objectives explained
Strong organizational and record-keeping skills
Experience carrying out retro-orbital bleeds and intravenous and intraperitoneal injections, isolation and preparation of mouse tissues for antibody staining, and analysis by flow cytometry
Experience with molecular biology techniques including primer design, PCR, and cloning
Experience with mammalian cell tissue culture and sterile technique #LI-SB1
Internal Number: 2019-34866
About Memorial Sloan-Kettering Cancer Center
As one of the world's premier cancer centers, Memorial Sloan-Kettering Cancer Center is committed to exceptional patient care, leading-edge research, and superb educational programs. The close collaboration between our physicians and scientists is one of our unique strengths, enabling us to provide patients with the best care available today as we work to discover more effective strategies to prevent, control, and ultimately cure cancer in the future. Our education programs train future physicians and scientists, and the knowledge and experience they gain at Memorial Sloan-Kettering has an impact on cancer treatment and the biomedical research agenda around the world.