(80%) Purify peptide-MHCII monomers for tetramer generation which are used as a flow cytometric staining tool to detect antigen specific CD4 T cells. This includes maintaining cultures of Drosophila Schneider (S2) cells, cloning expression constructs, transfection of S2 cells, selection and scale up, induction of the cells to express pMHCII-biotin molecules (monomers), purifying these monomers from the supernatant, determining the purity on a coomassie gel. Making stock buffers and reagents and washing dishes needed for these procedures.
(20%) Maintaining detailed records of all experiments, procedures and production details in laboratory notebooks and digitally on the Jenkins lab server.
All required qualifications must be documented on application materials.
-B.S. or equivalent in Biology, Microbiology or related field and one year of professional experience working in an academic research laboratory.; or a combination of related education and work experience to total five years.
Preferred Qualifications: - Minimum of at least two year’s experience in an academic research laboratory. - Experience with basic molecular biology procedures, such as DNA isolation, PCR, basic cloning techniques, sequence analysis, agarose gel electrophoresis, SDS-PAGE and western blots - Experience with Recombinant protein production and purification (affinity chromatography) - Experience with basic cell culture procedures – bacterial, insect and mammalian - The individual should be highly self-motivated and willing to work flexible hours.
Internal Number: 331735
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.