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The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.
A laboratory in the UW Department of Biochemistry currently has an outstanding opportunity for a Research Scientist/Engineer 1 for studies of membrane protein function and structure. The overall project combines high-throughput saturation mutagenesis approaches with structural analyses and biochemical reconstitution to understand the mechanisms of membrane docking and fusion.
The Research Scientist/Engineer will:
Clone and purify proteins from E. coli, S. cerevisiae, and other microbes, using affinity and classical chromatography techniques.
Perform biochemical reconstitution experiments to test the functions of membrane-resident proteins, using fluorescence (FRET and anisotropy) techniques
Structurally characterize these protein complexes using electron cryo microscopy and mass spectrometry approaches.
Bachelor’s degree in Chemistry, Biochemistry, Biomedical Engineering, or similar discipline, with at least one year of prior laboratory research experience.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Other required qualifications include:
Strong organizational skills.
Ability and ambition to learn new techniques and approaches.
Strong quantitative skills, with computer programming experience preferred.
Ability and enthusiasm to work as part of a team. •Intellectual integrity.
Experience with protein expression and purification including liquid chromatography.
Practical understanding of recombinant DNA and cloning strategies and tactics, including but not limited to polycistronic vector design and Gibson cloning.
Experience with PyMol or Chimera molecular modeling packages. Experience using Python and R languages in a life science setting.
Ability to learn and integrate novel wet bench and in silico methods.
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Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.